Please use this identifier to cite or link to this item: http://imsear.hellis.org/handle/123456789/169102
Title: Induction of Apoptosis in MCF-7 Cells by Methanolic Extract of Clitoria Ternatea L.
Authors: Shivaprakash, Priyanka
Balaji, Kyathegowdanadoddi Srinivasa
Chandrashekara, Kagepura Thammaiah
Rangappa, Kanchugarakoppal Subbegowda
Jayarama, Shankar
Keywords: Clitoria ternatea
Apoptosis
MTT assay
DNA fragmentation
Caspase-3
Issue Date: Oct-2015
Citation: Shivaprakash Priyanka, Balaji Kyathegowdanadoddi Srinivasa, Chandrashekara Kagepura Thammaiah, Rangappa Kanchugarakoppal Subbegowda, Jayarama Shankar. Induction of Apoptosis in MCF-7 Cells by Methanolic Extract of Clitoria Ternatea L. International Journal of Applied Biology and Pharmaceutical Technology. 2015 Oct-Dec; 6(4): 80-86.
Abstract: Aim: Investigation of apoptosis induction by methanolic extract of Clitoria ternatea L. flower against multiple cancer cell lines. Main Methods: In the present study cytotoxic activity of Clitoria ternatea L flower was determined using MTT cell viability assay. The induction of cell death/apoptosis was evaluated by light microscopy, DNA fragmentation and caspase-3 enzyme activation. Key Findings: The methanolic extract from C. ternatea (MECT) showed cytotoxic activity against several cancer cell lines. The most potent activity exhibited by MECT was against MCF-7 breast carcinoma cells with an IC50 value of 27.2 ± 2.6 μg/mL. Light microscopic evaluation clearly indicated the apoptotic morphology of MECT treated cells. Treatment of MCF-7 cells with various MECT concentrations resulted in growth inhibition and induction of apoptosis as indicated by DNA fragmentation and caspase-3 enzyme activation. Significance: The current report strongly suggests the pro-apoptotic properties of C. ternatea flowers. Our findings demonstrate that C. ternatea phyto-constituents may have beneficial applications in the field of anti-cancer drug discovery.
URI: http://imsear.hellis.org/handle/123456789/169102
ISSN: 0976-4550
Appears in Collections:International Journal of Applied Biology and Pharmaceutical Technology

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